Anti-SIRT2 antibody
| 英文名称 | SIRT2 |
| 中文名称 | 沉默调节相关蛋白2抗体 |
| 别 名 | FLJ35621; FLJ37491; NAD dependent deacetylase sirtuin 2; NAD-dependent deacetylase sirtuin-2; Regulatory protein SIR2 homolog 2; Silencing information regulator 2 like; Silent information regulator 2; Silent mating type information regulation 2; Silent mating type information regulation 2 homolog; SIR 2; SIR2; SIR2 like; SIR2 like protein 2; Sir2 related protein type 2; SIR2-like protein 2; SIR2L; SIR2L2; SIRT 2; SIRT2; SIRT2_HUMAN; Sirtuin (silent mating type information regulation 2 homolog) 2 (S.cerevisiae); Sirtuin 2; Sirtuin type 2; Sirtuin2. |
DATASHEET
Host:Rabbit
Target Protein:SIRT2
IR:Immunogen Range:261-360/389
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:22933
Swiss Prot:Q8IXJ6
Source:KLH conjugated synthetic peptide derived from human SIRT2:261-360/389
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class I of the sirtuin family. Several transcript variants are resulted from alternative splicing of this gene. [provided by RefSeq, Jul 2010]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
IHC-P(1:400-800)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Cerebellum (Mouse) Lysate at 40 ug
Lane 3: Cerebrum (Rat) Lysate at 40 ug
Lane 4: Cerebellum (Rat) Lysate at 40 ug
Primary: Anti-SIRT2 (bs-24319R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43/37 kD
Observed band size: 43 kD
Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SIRT2) Polyclonal Antibody, Unconjugated (bs-24319R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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