Anti-N-Cadherin antibody (bs-55138R)

Anti-N-Cadherin antibody

DATASHEET

Host:Rabbit

Target Protein:N-Cadherin

IR:Immunogen Range:750-850-905

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:1000

Swiss Prot:P19022

Source:KLH conjugated synthetic peptide derived from human N-Cadherin:750-850-905 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Background:This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a calcium dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. The protein functions during gastrulation and is required for establishment of left-right asymmetry. At certain central nervous system synapses, presynaptic to postsynaptic adhesion is mediated at least in part by this gene product.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-1000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: Hela (Human) Cell Lysate at 25 ug
Lane 2: N-Cadherin knockout (KO) HeLa (Human) Cell Lysate at 25 ug
Primary: Anti-N-Cadherin (bs-55138R) at 1/1000 dilution
Secondary: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution
Predicted band size: 130 kD
Observed band size: 135 kD

Sample:
Lane 1: Heart (Mouse) Lysate at 40 ug
Lane 2: A549 (Human) Cell Lysate at 30 ug
Primary: Anti-N-Cadherin (bs-55138R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 130 kD
Observed band size: 130 kD