Anti-AIF1/Iba1 antibody
| 英文名称 | AIF1/Iba1 |
| 中文名称 | 离子钙接头蛋白抗体 |
| 别 名 | AIF 1; AIF1; AIF1 protein; IBa1; iba-1; IBa 1; allograft inflammatory factor 1; Allograft inflammatory factor 1 splice variant G; balloon angioplasty responsive transcription; BART 1; G1; G1 putative splice variant of allograft inflamatory factor 1; IBA 1; IBA1; interferon gamma responsive transcript; Ionized calcium binding adapter molecule 1; ionized calcium-binding adapter molecule; IRT 1; IRT1; Microglia response factor; MRF1; Protein G1. |
DATASHEET
Host:Rabbit
Target Protein:AIF1/Iba1
IR:Immunogen Range:6-36/147
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:199
Swiss Prot:P55008
Source:KLH conjugated synthetic peptide derived from human AIF1:6-36/147
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Allograft Inflammatory Factor-1 (AIF1)or ionized calcium-binding adaptor molecule 1 (Iba1) is expressed selectively in microglia/macrophages and is a Ca2+-binding peptide produced by activated monocytes and microglial cells. It has been suggested that AIF1 expression is associated with chronic inflammatory processes. AIF1 is expressed by activated monocytes and might participate in a variety of pathogenic processes in the mammalian brain and in chronic transplant rejection. It has been shown to be expressed early and persistently in chronically rejecting cardiac allografts but not in cardiac syngrafts and host hearts.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:2000)
ELISA(1:5000-10000)
IHC-P(1:50-100)
Cross Reactive Species:Human
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For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Lane 1: HL-60 (Human) Cell Lysate at 30 ug
Lane 2: U937 (Human) Cell Lysate at 30 ug
Primary: Anti-AIF1/Iba1 (bs-34006R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kD
Observed band size: 20 kD
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (AIF1) Polyclonal Antibody, Unconjugated (bs-34006R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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