Anti-Bcl-2 antibody (bs-33411R)

Anti-Bcl-2 antibody 

DATASHEET

Host:Rabbit

Target Protein:Bcl-2

IR:Immunogen Range:51-150/239

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:596

Swiss Prot:P49950

Source:KLH conjugated synthetic peptide derived from human Bcl-2 :51-150/239 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

Background:The Bcl-2 gene was isolated at the chromosomal breakpoint of t(14;18)-bearing follicular B cell lymphomas(1,2).Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal (3,5).Bcl-2 appears to function in several subcellular locations yet lacks any known motifs that would confer insight into its mechanism of action (6,7).A more recently identified protein,designated Bax p21(i.e., Bcl-associated X protein ),has extensive amino acid homology with Bcl-2 and both homodimerizes and forms heterodimers with Bcl-2(8). Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line and Bax also counters the death repressor activty of Bcl-2(8).

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)

Cross Reactive Species:Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: Spleen (Rat) Lysate at 40 ug
Lane 2: Bone (Rat) Lysate at 40 ug
Primary:
Anti- Bcl-2 (bs-33411R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 26 kD

Sample:
Lane 1: Spleen (Mouse) Lysate at 40 ug
Lane 2: Lymph node (Mouse) Lysate at 40 ug
Lane 3: Bone (Mouse) Lysate at 40 ug
Lane 4: Thyroid gland (Mouse) Lysate at 40 ug
Lane 5: Spleen (Rat) Lysate at 40 ug
Primary:
Anti- Bcl-2 (bs-33411R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 26 kD
Observed band size: 26 kD