Anti-STK4 antibody
| 英文名称 | STK4 |
| 中文名称 | 蛋白激酶MST抗体 |
| 别 名 | Kinase responsive to stress; Krs2; Mammalian STE20 like protein kinase 1; Mammalian STE20-like protein kinase 1; Mammalian sterile 20 like 1; MST-1; Serine/threonine kinase 4; Serine/threonine protein kinase Krs 2; Serine/threonine-protein kinase 4; Serine/threonine-protein kinase Krs-2; STE20 like kinase MST1; STE20-like kinase MST1; MST1; STK4_HUMAN; TIIAC; YSK3. |
DATASHEET
Host:Rabbit
Target Protein:STK4
IR:Immunogen Range:303-487/487
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:6789
Swiss Prot:Q13043
Source:Recombinant human STK4:303-487/487
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Background:The protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process. [provided by RefSeq, Jul 2008]
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
Cross Reactive Species:Human
Mouse
Rat
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
Lane 1: Hela (Human) Cell Lysate at 25 ug
Lane 2: STK4 knockout (KO) Hela (Human) Cell Lysate at 25 ug
Primary: Anti-STK4 (bs-55209R) at 1/1000 dilution
Secondary: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution
Predicted band size: 60 kD
Observed band size: 57 kD
Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug
Lane 2: Testis (Mouse) Lysate at 40 ug
Lane 3: Spleen (Mouse) Lysate at 40 ug
Lane 4: Lymph node (Mouse) Lysate at 40 ug
Lane 5: NIH/3T3 (Mouse) Cell Lysate at 30 ug
Lane 6: Cerebrum (Rat) Lysate at 40 ug
Lane 7: Testis (Rat) Lysate at 40 ug
Lane 8: Hela (Human) Cell Lysate at 30 ug
Lane 9: Jurkat (Human) Cell Lysate at 30 ug
Lane 10: HL60 (Human) Cell Lysate at 30 ug
Lane 11: Molt-4 (Human) Cell Lysate at 30 ug
Primary: Anti-STK4 (bs-55209R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 60 kD
Observed band size: 58 kD
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