Anti-PADI4 antibody (bs-0318R)

Anti-PADI4 antibody

DATASHEET

Host:Rabbit

Target Protein:PADI4

IR:Immunogen Range:401-500/663

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:23569

Swiss Prot:Q9UM07

Source:KLH conjugated synthetic peptide derived from human PADI4:401-500/663 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:PADI4 (peptidyl arginine deiminase, type IV) catalyzes the deimination of arginine residues of proteins. Down-regulates histone H3 and H4 arginine methylation, both by preventing arginine methylation by CARM1 and HRMT1L2/PRMT1 and by converting methylarginine to citrulline. subcellular location at cytoplasmic granules. Belongs to the protein arginine deiminase family. This gene may play a role in granulocyte and macrophage development leading to inflammation and immune response.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (human breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PADI4) Polyclonal Antibody, Unconjugated (bs-0318R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.