Anti-Phospho-CHK2 (Thr26) antibody
| 英文名称 | Phospho-CHK2 (Thr26) |
| 中文名称 | 磷酸化细胞周期检测点激酶2抗体 |
| 别 名 | Chk2 (phospho T26); p-Chk2 (phospho T26); bA444G7; CHK2 checkpoint homolog; CHK2_HUMAN; Serine/threonine-protein kinase Chk2; CDS 1; CDS1; Checkpoint kinase 2; Checkpoint like protein CHK2; Chek 2; Chek2; Chk 2; CHK2 checkpoint homolog (S. pombe); CHK2 checkpoint homolog; HuCds 1; HuCds1; LFS 2; LFS2; PP1425; RAD 53; RAD53; Rad53 homolog; Serine/threonine protein kinase Chk2. |
DATASHEET
Host:Rabbit
Target Protein:Phospho-CHK2 (Thr26)
IR:Immunogen Range:SV(p-T)Q
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:11200
Swiss Prot:O96017
Source:KLH conjugated Synthesised phosphopeptide derived from human CHK2 around the phosphorylation site of Thr26:SV(p-T)Q
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Chk2 is a serine/threonine kinase involved in the control of cell cycle checkpoints, and may also participate in transduction of the DNA damage and replicational stress signals. Chk2 is the mammalian ortholog of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases. The amino-terminal domain of Chk2 contains a series of seven serine and threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50 and Thr68) followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases. Indeed, after DNA damage by ionizing radiation (IR), UV irradiation or hydroJNCurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR. The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 on residues Thr383 and Thr387 in the activation loop of the kinase domain. Chk2 inhibits CDC25C phosphatase by phosphorylating it on Ser-216, preventing the entry into mitosis. This kinase may have a role in meiosis as well. Kinase activity is up regulated by autophosphorylation and the protein is rapidly phosphorylated in response to DNA damage and to replication block.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
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For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: U937(Human) Cell Lysate at 40 ug
Primary: Anti-p-CHK2 (Thr26)(bs-13909R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 61kD
Observed band size: 61kD
Sample: K562 (Human)Cell Lysate at 40 ug
Primary: Anti-p-CHK2 (Thr26)(bs-13909R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 61kD
Observed band size: 61kD
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