Anti-IAA antibody (bs-0902R)

Anti-IAA antibody 

DATASHEET

Host:Rabbit

Target Protein:IAA

IR:Immunogen Range:

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:87-51-4

Swiss Prot:N/A

Source:KLH conjugated with IAA: 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Indole-3-acetic acid, also known as IAA, is a heterocyclic compound that is an phytohormones called auxins. This colourless solid is probably the most important plant auxin. The molecule is derived from indole, containing a carboJNCmethyl group (acetic acid). IAA has many different effects, as all auxins do, such as inducing cell elongation and cell division with all subsequent results for plant growth and development. There are less expensive and metabolically stable synthetic auxin analogs on the market for use in horticulture, such as indole-3-butyric acid (IBA) and 1-naphthaleneacetic acid (NAA).

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Indole-3-AceticAcid.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Tissue/cell: Peach seed tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-IAA Polyclonal Antibody, Unconjugated(bs-0902M) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Mouse IgG, PE-Cy5 conjugated(bs-0296G-PE-Cy5)used at 1:200 dilution for 40 minutes at 37°C.