Anti-HSP105 antibody (bs-1387R)

Anti-HSP105 antibody

DATASHEET

Host:Rabbit

Target Protein:HSP105

IR:Immunogen Range:201-350/858

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:10808

Swiss Prot:Q92598

Source:KLH conjugated synthetic peptide derived from human HSP105:201-350/858 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Heat Shock Proteins are ubiquitously expressed in all organisms. They are induced when a cell undergoes various types of environmental stresses like heat, cold and oJNCgen deprivation. They are characterized by their role as molecular chaperones.
Heat shock protein (HSP) 105 exists as two isoforms; alpha and beta which belong to the HSP105/HSP110 protein family. HSP105 acts as both a chaperone to prevent thermal aggregation of proteins and as a regulator of mammalian cells. The HSP105 isoforms are found in the cytoplasm but not in the nucleoli under non-stressed and stressed conditions.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-HSP105 Polyclonal Antibody, Unconjugated(bs-1387R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining