Anti-FDC-SP antibody (bs-1766R)

Anti-FDC-SP antibody

DATASHEET

Host:Rabbit

Target Protein:FDC-SP

IR:Immunogen Range:18-85/85

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:260436

Swiss Prot:Q5R1M6

Source:KLH conjugated synthetic peptide derived from human FDC-SP:18-85/85 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Follicular dendritic cells are cells that facilitate antigen recognition by B cells in follicles. This kind of dendritic cell is not bone marrow derived and is not a kind of blood cell. It is purely a resident of the follicles of secondary lymphoid organs. B cells form germinal centers around follicular dendritic cells in lymphoid organs. Dendritic cells form from monocytes, white blood cells which circulate in the body and, depending on the right signal, can turn into dendritic cells or macrophages. The monocytes in turn are formed from stem cells in the bone marrow.
In normal tissue this antibody identifies dendritic cells and a proportion of B lymphocytes. Evidence in pathological tissue and functional studies suggests it binds to an epitope expressed by antigen presenting cells. Within the macrophage/dendritic cell populations the epitope seen by RFD1 is coexpressed with RFD7 by a subset of cells that exhibit suppressive activity on T cell stimulation.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (mouse lymphoid); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FDC-SP) Polyclonal Antibody, Unconjugated (bs-1766R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.