Anti-ABCG4 antibody (bs-1727R)

Anti-ABCG4 antibody

DATASHEET

Host:Rabbit

Target Protein:ABCG4

IR:Immunogen Range:551-646/646

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:64137

Swiss Prot:Q9H172

Source:KLH conjugated synthetic peptide derived from human ABCG4:551-646/646 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:he ATP binding cassette (ABC) superfamily of membrane transporters is one of the largest protein classes known, and counts for numerous proteins involved in trafficking of biological molecules across membranes. ABCG4 protein is highly expressed in both human and mouse brain, it is a 646aa molecule in human (chr 11q23) and mouse. It is an integral membrane protein may be involved in macrophage lipid homeostasis. The ABCG4 protein’s abundant expression in brain and close evolutionary relationship to the other members of the subfamily suggests a potential role in cholesterol transport.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (ABCG4) Polyclonal Antibody, Unconjugated (bs-1727R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.