Anti-Phospho-Estrogen Receptor alpha (Tyr537) antibody
| 英文名称 | Phospho-Estrogen Receptor alpha (Tyr537) |
| 中文名称 | 磷酸化雌激素受体α抗体 |
| 别 名 | Estrogen Receptor alpha (phospho Y537); p-Estrogen Receptor alpha (phospho Y537); ER alpha (Phospho-Tyr537); ER alpha (Phospho-Y537); Phospho-ER alpha (Y537); Phospho-ER alpha (Tyr537); p-ER alpha (Tyr537); p-ER alpha (Y537); Phospho-Estrogen Receptor alpha (Y537); Phospho-Estrogen Receptor alpha (Tyr537); Estradiol receptor; Estrogen receptor alpha; Estradiol Receptor-alpha; Estrogen Receptor 1; Atherosclerosis, susceptibility to, included; DKFZp686N23123; ER Alpha; ER; ER-alpha; ERalpha; ER[a]; Era; ESR; ESR1; ESR1_HUMAN; ESR2; ESRA; Estr; Estrogen receptor 1 (alpha); Estrogen resistance, included; HDL cholesterol, augmented response of, to hormone replacement, included; Myocardial infarction, susceptibility to, included; NR3A1; Nuclear receptor subfamily 3 group A member 1; OTTHUMP00000017718; OTTHUMP00000017719; RNESTROR. |
DATASHEET
Host:Rabbit
Target Protein:Phospho-Estrogen Receptor alpha (Tyr537)
IR:Immunogen Range:PL(p-Y)DL
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:2099
Swiss Prot:P03372
Source:KLH conjugated synthetic peptide derived from human ER-Alpha:PL(p-Y)DL
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Estrogen and progesterone receptor are members of a family of transcription factors that are regulated by the binding of their cognate ligands. The interaction of hormone-bound estrogen receptors with estrogen responsive elements(EREs) alters transcription of ERE-containing genes. The carboJNC terminal region of the estrgen receptor contains the ligand binding domain, the amino terminus serves as the transactivation domain, and the DNA binding domain is centrally located. Two forms of estrogen receptor have been identified, ER Alpha and ER Beta. ER Alpha and ER Beta have been shown to be differentially activated by various ligands. The biological response to progesterone is mediated by two distinct forms of the human progesterone receptor (hPR-A and hPR-B), which arise from alternative splicing. In most cells, hPR-B functions as a transcriptional activator of progesterone-responsive gene, whereas hPR-A function as a transcriptional inhibitor of all steroid hormone receptors.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(0.2μg /test)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Blank control (blue line): MCF7 (blue).
Primary Antibody (green line): Rabbit Anti-Phospho-Estrogen Receptor alpha (Tyr537) antibody (bs-1643R)
Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 80% ethanol (Overnight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min on ice. Cells stained with Primary Antibody for 30 min at room temperature. The cells were then incubated in 1 X PBS/2%BSA/10% goat serum to block non-specific protein-protein interactions followed by the antibody for 15 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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