Anti-Profilin 2 antibody (bs-1875R)

Anti-Profilin 2 antibody

DATASHEET

Host:Rabbit

Target Protein:Profilin 2

IR:Immunogen Range:51-140/140

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:5217

Swiss Prot:Q9EPC6

Source:KLH conjugated synthetic peptide derived from human Profilin 2:51-140/140 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Profilin 2 is a ubiquitous actin monomer-binding protein belonging to the profilin family. It is thought to regulate actin polymerization in response to extracellular signals by binding to actin (1:1 ratio) and so affecting the structure of the cytoskeleton. At high concentrations, profilin prevents the polymerization of actin whereas it enhances it at low concentrations. By binding to PIP2, it inhibits the formation of IP3 and DG. Highly expressed in skeletal muscle, kidney and brain and less strongly in placenta, heart, liver and lung.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Pig
Cow
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Profilin 2) Polyclonal Antibody, Unconjugated (bs-1875R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.