Anti-Vitronectin antibody (bs-1932R)

Anti-Vitronectin antibody

DATASHEET

Host:Rabbit

Target Protein:Vitronectin

IR:Immunogen Range:381-478/478

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:7448

Swiss Prot:P04004

Source:KLH conjugated synthetic peptide derived from human Vitronectin:381-478/478 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Vitronectin (also known as serum spreading factor, S protein of complement or epibolin) is one of the major multifunctional cell adhesive glycoproteins in mammalian plasma and serum. It is a monomeric acidic glycoprotein detected as a mixture of 75 kD and 65 kD polypeptides. Vitronectin binds to heparin, collagen, streptococci and variety of cultured cells. It also acts as an inhibitor of the complement cascade by binding to the C5b9 complex. Vitronectin promotes cell adhesion and spreading by binding through its cell attachment tripeptide Arg-Gly-Asp (RG-D), activity which is mediated by several different integrin receptors. Apart from the significance for identifying the molecule in the above situations, it also plays an important role in events such as embryonal development, deposition of vitronectin in a number of fibrotic disease states, carcinomas and metastases.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Vitronectin) Polyclonal Antibody, Unconjugated (bs-1932R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.