Anti-phospho-GEF H1 (Ser886) antibody (bs-2201R)

Anti-phospho-GEF H1 (Ser886) antibody

DATASHEET

Host:Rabbit

Target Protein:phospho-GEF H1 (Ser886)

IR:Immunogen Range:RR(p-S)LP

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:9181

Swiss Prot:Q92974

Source:KLH conjugated Synthesised phosphopeptide derived from human Arhgef2 around the phosphorylation site of Ser886:RR(p-S)LP 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate rho-dependent signals. Alternatively spliced transcript variants encoding different isoforms have been identified.[provided by RefSeq, Jun 2009]

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (phospho-GEF H1 (Ser886)) Polyclonal Antibody, Unconjugated (bs-2201R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.