Anti-phospho-eIF4EBP1 (Thr37 + Thr46) antibody
| 英文名称 | phospho-eIF4EBP1 (Thr37 + Thr46) |
| 中文名称 | 磷酸化eIF4E结合蛋白1抗体 |
| 别 名 | eIF4EBP1 (phospho T37/46); eIF4EBP1 (phospho Thr37/46); p-eIF4EBP1 (Thr37/46); eIF4EBP1; Eukaryotic translation initiation factor 4E binding protein 1; Eukaryotic translation initiation factor 4E-binding protein 1; 4E BP1; 4EBP1; BP 1; BP1; eIF4E binding protein 1; eIF4E-binding protein 1; Eukaryotic translation initiation factor 4E binding protein 1; MGC4316; PHAS I; PHASI; PHAS-I; PHAS; 4E-BP1; Phosphorylated heat- and acid-stable protein regulated by insulin 1; Phosphorylated heat and acid stable protein regulated by insulin 1; 4EBP1_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:phospho-eIF4EBP1 (Thr37 + Thr46)
IR:Immunogen Range:ST(p-T)PGGTLFST(p-T)P
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:1978
Swiss Prot:Q13541
Source:KLH conjugated Synthesised phosphopeptide derived from human 4EBP1 around the phosphorylation site of Thr37/46:ST(p-T)PGGTLFST(p-T)P
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation. [provided by RefSeq, Jul 2008].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-eIF4EBP1(Thr37+Thr46) Polyclonal Antibody, Unconjugated(bs-3019R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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