Anti-Phospho-Bcr (Tyr177) antibody
| 英文名称 | Phospho-Bcr (Tyr177) |
| 中文名称 | 磷酸化T淋巴细胞受体抗体 |
| 别 名 | Bcr (phospho Y177); Bcr(Phospho-Tyr177); ALL; BCR 1; BCR/ABL FUSION GENE INCLUDED; BCR/FGFR1 FUSION GENE INCLUDED; BCR/PDGFRA FUSION GENE INCLUDED; BCR1; breakpoint cluster region; CML; PHL; BCR_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:Phospho-Bcr (Tyr177)
IR:Immunogen Range:PF(p-Y)VN
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:613
Swiss Prot:P11274
Source:KLH conjugated Synthesisedphosphopeptide derived from human Bcr around the phosphorylation site of Tyr177:PF(p-Y)VN
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The breakpoint cluster region protein (Bcr) is best know to be involved in genomic translocation with fusion partner Abl (Cbr-Abl) causing chronic myelogenous leukemia (CML). This 160 kDa protein contains a serine/threonine kinase domain, an SH2 binding domain, a GTP/GDP exchange domain and a C-term domain which functions as a GTPase activating protein for p21rac and CDC42. Additionally, Bcr is involved in signal transduction and can down regulate Ras mediated cell signaling.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Pig
Rabbit
Guinea Pig
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample:
K562(Human) Cell Lysate at 30 ug
Primary: Anti-Phospho-Bcr (Tyr177) (bs-3067R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 143 kD
Observed band size: 137 kD
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-VNN1 antibody (bs-3067R-AF488)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.
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