Anti-phospho-Cortactin (Thr466) antibody (bs-3100R)

Anti-phospho-Cortactin (Thr466) antibody

DATASHEET

Host:Rabbit

Target Protein:phospho-Cortactin (Thr466)

IR:Immunogen Range:YA(p-T)EA

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:2017

Swiss Prot:Q14247

Source:KLH conjugated Synthesised phosphopeptide derived from human Cortactin around the phosphorylation site of Thr466:YA(p-T)EA 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq, May 2010]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Hela(Human) Cell Lysate at 30 ug
MCF-7(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-Cortactin (Thr466)(bs-3100R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 61 kD
Observed band size: 61/57 kD