Anti-phospho-Ephrin B (Ser300) antibody (bs-3128R)

Anti-phospho-Ephrin B (Ser300) antibody

DATASHEET

Host:Rabbit

Target Protein:phospho-Ephrin B (Ser300)

IR:Immunogen Range:EP(p-S)DI

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:1947

Swiss Prot:P52799

Source:KLH conjugated Synthesised phosphopeptide derived from human Ephrin B around the phosphorylation site of Ser300:EP(p-S)DI 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Ephrin B proteins are thought to play key roles in cellular functions as diverse as neuronal migration and blood vessel development. Ephrin B molecules expressed at the membrane surface bind to the Ephrin B family receptors on target cells during cell to cell contact. This interaction leads to cell signaling in the target cell but also generates a reverse signal in the cell expressing Ephrin B on its surface. This reverse signaling event is thought to be critical for vessel maturation and neuronal development. Importantly, tyrosine phosphorylation of Ephrin B is thought to be a critical component of this reverse signaling event. Recent work demonstrated that Tyr331 of Ephrin B was phosphorylated in HEK293 cells after stimulation by the soluble Ephrin B2 receptor tyrosine kinase.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
LOVO(Human) Cell Lysate at 30 ug
Primary: Anti-phospho-Ephrin B (Ser300) (bs-3128R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 38 kD