Anti-Phospho-GSK3 alpha (Ser21) antibody
| 英文名称 | Phospho-GSK3 alpha (Ser21) |
| 中文名称 | 磷酸化糖原合酶激酶3α抗体 |
| 别 名 | GSK3 alpha (phospho S21); p-GSK3 alpha (phospho S21); GSK3a(Phospho-Ser21); P-GSK3 alpha(Ser21); Gsk3a; GSK 3 alpha; GSK-3 alpha; GSK3 alpha; Glycogen synthase kinase-3 alpha; GSK 3 alpha; DKFZp686D0638; GSK 3A; GSK3A; GSK3alpha; GSK3A_HUMAN; Glycogen synthase kinase 3 alpha; Serine/threonine-protein kinase GSK3A. |
DATASHEET
Host:Rabbit
Target Protein:Phospho-GSK3 alpha (Ser21)
IR:Immunogen Range:RT(p-S)SF
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:2931
Swiss Prot:P49840
Source:KLH conjugated synthesised phosphopeptide derived from human GSK3 alpha around the phosphorylation site of Ser21:RT(p-S)SF
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Glycogen synthase kinase 3 alpha belongs to the Ser/Thr family of protein kinases, Cdc2/cdkx subfamily; GSK3 subsubfamily. It is implicated in the hormonal control of several regulatory proteins including glycogen synthase, myb, and the transcription factor c jun. GSK3 phosphorylates glycogen synthase and thereby inactivates it. Insulin stimulates the dephosphorylation of glycogen synthase at the sites phosphorylated by GSK3 and subsequently inhibits GSK3 acutely leading to the stimulation of glycogen synthesis. GSK3 signaling is performed by two isoforms, GSK3 alpha and GSK3 beta. The two isoforms share 97% sequence similarity within their catalytic domains. GSK3 has also been shown to play a role in protein synthesis, cell adhesion, cell proliferation, cell differentiation, microtubule dynamics and cell motility.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (mouse testis tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (P-GSK3 alpha (Ser21)) Polyclonal Antibody, Unconjugated (bs-3161R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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