Anti-Cardiac Troponin T antibody
| 英文名称 | Cardiac Troponin T |
| 中文名称 | 心肌特异性肌钙蛋白T抗体 |
| 别 名 | Cardiac muscle troponin T; Cardiomyopathy dilated 1D (autosomal dominant); Cardiomyopathy hypertrophic 2; CMD1D; CMH2; CMPD2; cTnT; LVNC6; MGC3889; OTTHUMP00000033864; OTTHUMP00000033865; OTTHUMP00000033866; OTTHUMP00000033867; OTTHUMP00000033870; OTTHUMP00000218095; RCM3; TNNT 2; TNNT2; TNNT2_HUMAN; TnTC; Troponin T cardiac muscle; Troponin T type 2 (cardiac); Troponin T type 2 cardiac; Troponin T, cardiac muscle; Troponin T2; Troponin T2 cardiac. |
DATASHEET
Host:Rabbit
Target Protein:Cardiac Troponin T
IR:Immunogen Range:201-298/298
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:7139
Swiss Prot:P45379
Source:KLH conjugated synthetic peptide derived from human Troponin T, cardiac muscle.:201-298/298
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq].
Size:100ul
Concentration:1mg/ml
Applications:IHC-P(1:50-200)
IHC-F(1:50-200)
Flow-Cyt(1μg /Test)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Rabbit
Guinea Pig
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Blank control:U-2OS.
Primary Antibody (green line): Rabbit Anti-TNNT2 antibody (bs-2804R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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