Anti-Phospho-LIMK1 (Thr508) + LIMK2(Thr505) antibody (bs-3247R)

Anti-Phospho-LIMK1 (Thr508) + LIMK2(Thr505) antibody

DATASHEET

Host:Rabbit

Target Protein:Phospho-LIMK1 (Thr508) + LIMK2(Thr505)

IR:Immunogen Range:RY(p-T)VV

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:3984

Swiss Prot:P53667

Source:KLH conjugated synthesised phosphopeptide derived from human LIMK1 around the phosphorylation site of Thr508:RY(p-T)VV 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:LIMK1 is a protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. LIMK1 may be involved in brain development; it is highly expressed in both adult and fetal nervous system. Detected ubiquitously throughout the different regions of adult brain, with highest levels in the cerebral cortex. Expressed to a lesser extent in heart and skeletal muscle.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (LIMK1) Polyclonal Antibody, Unconjugated (bs-3247R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.