Anti-Phospho-Stathmin 1 (Ser16) antibody (bs-3431R)

Anti-Phospho-Stathmin 1 (Ser16) antibody

DATASHEET

Host:Rabbit

Target Protein:Phospho-Stathmin 1 (Ser16)

IR:Immunogen Range:RA(p-S)GQ

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:3925

Swiss Prot:P16949

Source:KLH conjugated synthesised phosphopeptide derived from human Stathmin around the phosphorylation site of Ser16:RA(p-S)GQ 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene belongs to the stathmin family of genes. It encodes a ubiquitous cytosolic phosphoprotein proposed to function as an intracellular relay integrating regulatory signals of the cellular environment. The encoded protein is involved in the regulation of the microtubule filament system by destabilizing microtubules. It prevents assembly and promotes disassembly of microtubules. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2009]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by microwave in sodium citratebuffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3%BSA) at RTfor 30min; Antibody incubation with (Stathmin 1 (Ser16)) Polyclonal/MonoclonalAntibody, Unconjugated (bs-3431R) at 1:400 overnight at 4℃,followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.