Anti-phospho-TOP2A (Ser1106) antibody
| 英文名称 | phospho-TOP2A (Ser1106) |
| 中文名称 | 磷酸化DNA拓普西异构酶Ⅱ抗体 |
| 别 名 | TOP2A (phospho Ser1106); TOP2A (phospho S1106); Topoisomerase II alpha (phospho S1106); DNA topoisomeraseⅡalpha; TOPO II; DNA topoisomerase 2-alpha; DNA topoisomerase II, alpha isozyme; TOP2; TP2A; DNA topoisomerase II 170 kD; DNA topoisomerase II alpha isozyme; DNA Topoisomerase2; TOP 2A; TopII alpha; Topo II alpha; Topoisomerase DNA II alpha 170kDa; Topoisomerase II alpha 170 kDa; TOP2A_HUMAN; Topo IIα; Topo-IIα; Topoisomerase II-α. |
DATASHEET
Host:Rabbit
Target Protein:phospho-TOP2A (Ser1106)
IR:Immunogen Range:EE(p-S)DN
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:7153
Swiss Prot:P11388
Source:KLH conjugated Synthesised phosphopeptide derived from human TOPO II Alpha around the phosphorylation site of Ser1106:EE(p-S)DN
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. [provided by RefSeq, Jul 2010]
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Dog
Pig
Sheep
Danio.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (human tonsil tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TOP2A (Ser1106)) Polyclonal Antibody, Unconjugated (bs-3451R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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