Anti-Phospho-PKC gamma (Thr514) antibody
| 英文名称 | Phospho-PKC gamma (Thr514) |
| 中文名称 | 磷酸化蛋白激酶C亚型G抗体 |
| 别 名 | PKC gamma (phospho T514); p-PKC gamma (phospho T514); PKC gamma (Phospho Thr514); PKC gamma (Phospho T514); p-PKC gamma (Thr514); p-PKC gamma (Thr514); PKCC; PKCG; PRKCG; Protein kinase C gamma; Protein kinase C gamma polypeptide; Protein kinase C gamma type; SCA 14; SCA14; KPCG_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:Phospho-PKC gamma (Thr514)
IR:Immunogen Range:TR(p-T)FC
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:5582
Swiss Prot:P05129
Source:KLH conjugated Synthesised phosphopeptide derived from human PKC gamma around the phosphorylation site of Thr514:TR(p-T)FC
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. There have been at least 12 different PKC isoforms identified in humans to date including alpha, beta I, beta II, gamma, delta, epsilon, zeta, eta, theta, iota, lambda, and mu. PKC gamma is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14).
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Pig
Cow
Horse
Rabbit
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-PKC gamma (Thr514)) Polyclonal Antibody, Unconjugated (bs-3729R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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