Anti-Phospho-Paxillin (Tyr31) antibody (bs-3744R)

Anti-Phospho-Paxillin (Tyr31) antibody

DATASHEET

Host:Rabbit

Target Protein:Phospho-Paxillin (Tyr31)

IR:Immunogen Range:TP(p-Y)SY

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:5829

Swiss Prot:P49023

Source:KLH conjugated Synthesised phosphopeptide derived from human Paxillin around the phosphorylation site of Tyr31:TP(p-Y)SY 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011]

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Cow
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (Mouse uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Phospho-Paxillin (Tyr31)) Polyclonal Antibody, Unconjugated (bs-3744R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.