Anti-ESM1 antibody (bs-3615R)

Anti-ESM1 antibody

DATASHEET

Host:Rabbit

Target Protein:ESM1

IR:Immunogen Range:51-150/184

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:11082

Swiss Prot:Q9NQ30

Source:KLH conjugated synthetic peptide derived from human ESM1:51-150/184 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:ESM1 is a secreted dermatan sulfate proteoglycan which is predominantly expressed in the endothelial cells in human lung and kidney tissues. Expression of ESM1 is regulated by cytokines. ESM1 may have a role in lung endothelial cell-leukocyte interactions. The transcript contains multiple polyadenylation and mRNA instability signals. Two transcript variants encoding different isoforms have been found for this gene.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Pig
Cow
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Tissue/cell: human kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ESM1 Polyclonal Antibody, Unconjugated(bs-3615R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining