Anti-CYP24A1 antibody (bs-3899R)

Anti-CYP24A1 antibody 

DATASHEET

Host:Rabbit

Target Protein:CYP24A1

IR:Immunogen Range:351-450/514

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:1591

Swiss Prot:Q07973

Source:KLH conjugated synthetic peptide derived from human CYP24A1:351-450/514 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:CYP24A1 (cytochrome P450, family 24, subfamily A, polypeptide 1) is a member of the cytochrome P450 superfamily. It is a mitochondrial enzyme responsible for inactivating vitamin D3 metabolites through the C-24 oxidation pathway. In regulating the level of vitamin D3, CYP24A1 plays a role in calcium homeostasis and the vitamin D endocrine system. CYP24A1 also has a role in maintaining calcium homeostasis. It catalyzes the NADPH-dependent 24-hydroJNClation of 25-hydroJNCvitamin D(3) in the presence of adrenodoxin and NADPH-adrenodoxin reductase.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (CYP24A1) Polyclonal Antibody, Unconjugated (bs-3899R) at 1:400 overnight at 4°C, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.