Anti-MYL9 antibody (bs-2380R)

Anti-MYL9 antibody

DATASHEET

Host:Rabbit

Target Protein:MYL9

IR:Immunogen Range:2-100/172

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:10398

Swiss Prot:P24844

Source:KLH conjugated synthetic peptide derived from human MYL9:2-100/172 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:MYL9 is the myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads. MYL9 protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for MYL9 and the deduced 172-amino acid protein is highly conserved, with only 3 differences between the human and chicken proteins. Light chain phosphorylation causes the folded monomeric form of myosin to extend and assemble into filaments. This observation established the involvement of the MYL9 in conformational transitions of smooth muscle myosin.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:200-1000)
ELISA(1:500-5000)
IHC-P(1:200-1000)
IHC-F(1:200-1000)
IF(1:200-1000)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (rat heart tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MYL9) Polyclonal Antibody, Unconjugated (bs-2380R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.