Anti-EHHADH antibody (bs-4058R)

Anti-EHHADH antibody

DATASHEET

Host:Rabbit

Target Protein:EHHADH

IR:Immunogen Range:631-723/723

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:1962

Swiss Prot:Q08426

Source:KLH conjugated synthetic peptide derived from human EHHADH:631-723/723 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:EHHADH is one of three preoxisomal enzymes of the classic peroxisomal Beta-oxidation cycle involved in the catabolism of free fatty acids.Most dietary long chain fatty acids are oxidized in the mitochondria, but very-long-chain fatty acids (VLCFAs) must be shortened first by peroxisomal Beta-oxidation prior to complete oxidation in mitochondria.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
ICC(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Cow
Horse
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (rat kidney tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EHHADH) Polyclonal Antibody, Unconjugated (bs-4058R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.