Anti-Insig1 antibody (bs-5074R)

Anti-Insig1 antibody

DATASHEET

Host:Rabbit

Target Protein:Insig1

IR:Immunogen Range:101-200/277

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:3638

Swiss Prot:O15503

Source:KLH conjugated synthetic peptide derived from human Insig1:101-200/277 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:OJNCsterols regulate cholesterol homeostasis through liver X receptor (LXR) and sterol regulatory element binding protein (SREBP) mediated signaling pathway. Insig1 is an insulin-induced gene. It encodes an endoplasmic reticulum (ER) membrane protein that plays a critical role in regulating cholesterol concentrations in cells. Insig1 binds to the sterol-sensing domains of SREBP cleavage-activating protein (SCAP) and HMG CoA reductase, and is essential for the sterol-mediated trafficking of the two proteins. It may also play a role in growth and differentiation of tissues involved in metabolic control, and may play a regulatory role during G0/G1 transition of cell growth.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Liver (Mouse) Lysate at 40 ug
Primary: Anti-Insig1 (bs-5074R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30 kD
Observed band size: 30 kD