Anti-Thyroglobulin antibody (bs-0291R)

Anti-Thyroglobulin antibody

DATASHEET

Host:Rabbit

Target Protein:Thyroglobulin

IR:Immunogen Range:full length

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:7038

Swiss Prot:P01266

Source:Thyroglobulin protein:full length 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Thyroglobulin is the glycoprotein precursor of the iodinated thyroid hormones thyroxine (T4) and triiodothyronine (T3). Thyroglobulin is obtained from the thyroid gland and exhibits the general properties of the globulins. The human thyroglobulin (hTG) is a high molecular weight glycoprotein (605 kDa) found in the thyroid follicular cells. It plays a central role in the uptake, incorporation, and regulated biosynthesis of thyroid hormones, T4 and T3. Thyroid disorders are, in large part, due to autoimmune origin, and anti thyroglobulin autoantibodies were the first factor to be discovered. Anti hTG is found in all thyroid autoimmune diseases (Hashimoto's thyroiditis, Graves' diseases), with the highest level observed in Hashimoto's thyroiditis. Anti hTG is also characteristic of thyroid cancer, and its determination can be used for the follow up of cancer patients.

Size:100ul

Concentration:2mg/1ml

Applications:ELISA(1:2000-10000)
IHC-P(1:200-1000)
IHC-F(1:200-1000)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Cat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Paraformaldehyde-fixed, paraffin embedded (rat ovary); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TG) Polyclonal Antibody, Unconjugated (bs-0291R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.