Anti-phospho-c-Abl (Thr735) antibody
| 英文名称 | phospho-c-Abl (Thr735) |
| 中文名称 | 磷酸化非受体酪氨酸激酶c-Abl抗体 |
| 别 名 | Abelson Murine Leukemia Viral Oncogene Homolog 1; Abelson murine leukemia viral v abl oncogene homolog 1; Abl 1; ABL; Abl protein; Abl1; Bcr/c abl oncogene protein; JTK 7; JTK7; p150 ; Proto oncogene tyrosine protein kinase ABL1; Transformation gene oncogene ABL; v abl Abelson murine leukemia viral oncogene homolog 1; v abl. |
DATASHEET
Host:Rabbit
Target Protein:phospho-c-Abl (Thr735)
IR:Immunogen Range:SV(p-T)LP
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:25
Swiss Prot:P00519
Source:KLH conjugated synthetic peptide derived from human c-Abl:SV(p-T)LP
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The ABL1 protooncogene encodes a cytoplasmic and nuclear protein tyrosine kinase that has been implicated in processes of cell differentiation, cell division, cell adhesion, and stress response. Activity of c-Abl protein is negatively regulated by its SH3 domain, and deletion of the SH3 domain turns ABL1 into an oncogene. The t(9;22) translocation results in the head-to-tail fusion of the BCR (MIM:151410) and ABL1 genes present in many cases of chronic myelogeneous leukemia. The DNA-binding activity of the ubiquitously expressed ABL1 tyrosine kinase is regulated by CDC2-mediated phosphorylation, suggesting a cell cycle function for ABL1. The ABL1 gene is expressed as either a 6- or 7-kb mRNA transcript, with alternatively spliced first exons spliced to the common exons 2-11. [provided by RefSeq].
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Rabbit
Guinea Pig
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by microwave in sodium citrate buffer (pH6.0) ; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (3% BSA) at RT for 30min; Antibody incubation with (phospho-c-Abl(Thr735)) Polyclonal Antibody, Unconjugated (bs-5183R) at 1:400 overnight at 4℃, followed by conjugation to the secondary antibody (labeled with HRP)and DAB staining.
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