Anti-phospho-ATF4 (Ser245) antibody (bs-5190R)

Anti-phospho-ATF4 (Ser245) antibody

DATASHEET

Host:Rabbit

Target Protein:phospho-ATF4 (Ser245)

IR:Immunogen Range:NR(p-S)LP

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:468

Swiss Prot:P18848

Source:KLH conjugated Synthesised phosphopeptide derived from human ATF4 around the phosphorylation site of Ser245:NR(p-S)LP 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:ATF4 is a transcription factor that was originally identified as a widely expressed mammalian DNA binding protein that could bind a tax-responsive enhancer element in the LTR of HTLV1. The encoded protein was also isolated and characterized as the cAMP-response element binding protein 2 (CREB2). The protein encoded by this gene belongs to a family of DNA-binding proteins that includes the AP1 family of transcription factors, cAMP-response element binding proteins (CREBs) and CREB-like proteins. These transcription factors share a leucine zipper region that is involved in protein-protein interactions, located C-terminal to a stretch of basic amino acids that functions as a DNA binding domain (referenced from Entrez gene).

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti- phospho-ATF4 (Ser245) (bs-5190R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 38 kD
Observed band size: 38 kD