Anti-phospho-Cdc25B (Ser186) antibody
| 英文名称 | phospho-Cdc25B (Ser186) |
| 中文名称 | 磷酸化细胞分裂周期蛋白25B抗体 |
| 别 名 | Cdc25B (Phospho-Ser186); Cdc25B (Phospho-S186); p-Cdc25B (Ser186); p-Cdc25B (S186); Cdc 25B; Cdc25b; Cdc-25b; CDC25HU2; Cdc25m2; Cell division cycle 25 homolog B; Cell division cycle 25B; Cell division cycle 25B isoform 1; Cell division cycle 25B isoform 2; Cell division cycle 25B isoform 3; Cell division cycle 25B isoform 4; Cell division cycle 25B isoform 5; Dual specificity phosphatase Cdc25B; M phase inducer phosphatase 2; M-phase inducer phosphatase 2; MPIP2_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:phospho-Cdc25B (Ser186)
IR:Immunogen Range:AA(p-S)SS
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:12531
Swiss Prot:P30306
Source:KLH conjugated synthesised phosphopeptide derived from human Cdc25B around the phosphorylation site of Ser149:AA(p-S)SS
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:CDC25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist. [provided by RefSeq, Jul 2008].
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Tissue/cell: Mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-Cdc258 Polyclonal Antibody, Unconjugated(bs-5244R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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