Anti-phospho-CREB-1 (Ser129) antibody (bs-5256R)

Anti-phospho-CREB-1 (Ser129) antibody

DATASHEET

Host:Rabbit

Target Protein:phospho-CREB-1 (Ser129)

IR:Immunogen Range:IL(p-S)RR

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:1385

Swiss Prot:P16220

Source:KLH conjugated Synthesised phosphopeptide derived from human CREB-1 around the phosphorylation site of Ser129:IL(p-S)RR 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The ATF/CREB family consists of transcription factors that function through binding to the cAMP responsive element (CRE) palindromic octanucleotide, TGACCTCA. The best characterized members of this gene family include CREB-1, CREB-2, ATF-1,ATF-2,ATF-3and ATF-4. these transcription factors share highly-related COOH terminal leucine zipper demerization and basic DNA bindings but are highly divergent in their amino terminal domains. Although each of the ATF/CREB proteins bind CREs in their homodimeric form, in cerain instances they also bind as heterodimers, both within the ATF/CREB family and with members of the AP-1 transcription factor family. It has recentlybeen shown that protein kinase A-mediated CREB phosphorylation results in its binding to a 265kDa nuclear protein designated CBP (CREB-binding protein), which may reprecent a CREB co-activator.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
Rat
Pig
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: A431 (Human) Cell Lysate at 30 ug
Lane 2: Liver (Mouse) Lysate at 40 ug
Lane 3: 293T (Human) Cell Lysate at 30 ug
Primary: Anti-phospho-CREB-1 (Ser129) (bs-5256R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 43 kD
Observed band size: 47 kD