Anti-phospho-ILK-1(Thr173) antibody
| 英文名称 | phospho-ILK-1(Thr173) |
| 中文名称 | 磷酸化整合素连接激酶1抗体 |
| 别 名 | ILK-1 (phospho T173); ILK-1 (phospho Thr173); p-ILK-1 (Thr173); 59 kDa serine/threonine protein kinase; 59 kDa serine/threonine-protein kinase; ILK-2; ILK_HUMAN; Integrin linked Kinase; Integrin-linked protein kinase; DKFZp686F1765; EC 2.7.11.1; ILK 1; ILK 2; ILK; ILK1; ILK2; Integrin linked Kinase 2; Integrin linked protein kinase; p59; p59ILK. |
DATASHEET
Host:Rabbit
Target Protein:phospho-ILK-1(Thr173)
IR:Immunogen Range:GT(p-T)RT
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:3611
Swiss Prot:Q13418
Source:KLH conjugated Synthesised phosphopeptide derived from human ILK-1 around the phosphorylation site of Thr173:GT(p-T)RT
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The ILK protein is important in different biological pathways such as cell adhesion, anchorage-dependent cell cycle progression, oncogenic transformation, and growth factor signaling. The kinase activity of ILK is low in non-activated cells; its activity is stimulated by cell-ECM interactions and by certain growth factors. 3 Negative regulation of ILK is mediated by two phosphatases: PTEN, a tumor suppressor lipid sphatase, and ILKAP, a PP2C protein phosphatase. In tumor cells that do not express PTEN protein, ILK is constitutively active.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
Sheep
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Sample: Raji Cell (Human) Lysate at 40 ug
Primary: Anti-phospho-ILK-1(Thr173) (bs-5443R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
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