Anti-phospho-MCAM (Tyr641) antibody (bs-5472R)

Anti-phospho-MCAM (Tyr641) antibody

DATASHEET

Host:Rabbit

Target Protein:phospho-MCAM (Tyr641)

IR:Immunogen Range:EK(p-Y)ID

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:4162

Swiss Prot:P43121

Source:KLH conjugated Synthesised phosphopeptide derived from human MCAM around the phosphorylation site of Tyr641:EK(p-Y)ID 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:MCAM (MUC18 antigen, CD146), a member of the immuoglobulin superfamily, is an intrinsic membrane glycoprotein of 110-120 kDa found on the surface of endothelial cells, bone marrow fibroblasts and various melanomas. MCAM (Melanoma adhesion molecule) has been used as a marker of tumor progression in human melanoma because expression in those tumors correlates strongly with poor prognosis and the development of metastic disease. In addition, a number of human T, B and myeloid leukemic cell lines seem to express MCAM. The close structural relationship with N-CAM and related molecules suggests that MCAM may be also a developmentally regulated cell adhesion.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Siha(Human) Cell Lysate at 30 ug
Primary: Anti- phospho-MCAM (Tyr641) (bs-5472R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 71 kD
Observed band size: 118 kD