Anti-phospho-MAPK3 (Tyr204) antibody
| 英文名称 | phospho-MAPK3 (Tyr204) |
| 中文名称 | 磷酸化丝裂原活化蛋白激酶3抗体 |
| 别 名 | ERK1 (phospho Y204); p-ERK1 (phospho Y204); MAPK3(phospho Y204); ERK/MAPK(phospho T202/Y204); p44/42 MAP Kinase(Phospho-Thr202); ERK 1; ERK; ERK-1; ERK1; Extracellular Signal Regulated Kinase 1; Extracellular signal related kinase 1; Extracellular signal-regulated kinase 1; HGNC6877; HS44KDAP; HUMKER1A; MAP kinase 1; MK03_RAT; MK03_HUMAN; MAP Kinase; MAP kinase isoform p44; MAPK 1; MAPK; MAPK1; MAPK3; MGC20180; Mitogen-activated protein kinase 1; OTTHUMP00000174538; OTTHUMP00000174541; p44 ERK1; p44 MAPK; p44-ERK1; p44-MAPK; P44ERK1; P44MAPK; |
DATASHEET
Host:Rabbit
Target Protein:phospho-MAPK3 (Tyr204)
IR:Immunogen Range:TE(p-Y)VA
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:50689
Swiss Prot:P21708
Source:KLH conjugated Synthesised phosphopeptide derived from rat MAPK3 around the phosphorylation site of Tyr204:TE(p-Y)VA
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Rabbit
Guinea Pig
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (phospho-MAPK3(Tyr204)) Polyclonal Antibody, Unconjugated (bs-5497R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructions and DAB staining.
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