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Anti-phospho-PKMYT1 (Thr495) antibody (bs-5531R)

Anti-phospho-PKMYT1 (Thr495) antibody (bs-5531R)

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Anti-phospho-PKMYT1 (Thr495) antibody

英文名称phospho-PKMYT1 (Thr495)
中文名称磷酸化蛋白激酶PKMYT1(Thr495)抗体
别    名PKMYT1(phospho T495);cdc2 inhibitory kinase; Membrane associated tyrosine and threonine specific cdc2 inhibitory kinase; Membrane-associated tyrosine- and threonine-specific cdc2-inhibitory kinase; MYT1; Myt1 kinase; PKMYT 1; Pkmyt1; PMYT1_HUMAN; Protein kinase membrane associated tyrosine/threonine 1; Protein kinase Myt1; DKFZp547K1610; FLJ20093.  

DATASHEET

Host:Rabbit

Target Protein:phospho-PKMYT1 (Thr495)

IR:Immunogen Range:ED(p-T)LD

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:9088

Swiss Prot:Q99640

Source:KLH conjugated Synthesised phosphopeptide derived from human PKMYT1 around the phosphorylation site of Thr495:ED(p-T)LD 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:This gene encodes a member of the serine/threonine protein kinase family. The encoded protein is a membrane-associated kinase that negatively regulates the G2/M transition of the cell cycle by phosphorylating and inactivating cyclin-dependent kinase 1. The activity of the encoded protein is regulated by polo-like kinase 1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Lane 1: MCF-7 (Human) Cell Lysate at 30 ug
Lane 2: A431 (Human) Cell Lysate at 30 ug
Lane 3: Molt-4 (Human) Cell Lysate at 30 ug
Lane 4: K562 (Human) Cell Lysate at 30 ug
Lane 5: U2os (Human) Cell Lysate at 30 ug
Lane 6: U251 (Human) Cell Lysate at 30 ug
Lane 7: A549 (Human) Cell Lysate at 30 ug
Primary: Anti-phospho-PKMYT1 (Thr495) (bs-5531R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 55 kD
Observed band size: 55 kD

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