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Anti-phospho-TRIM25 (Ser100) antibody (bs-5615R)

Anti-phospho-TRIM25 (Ser100) antibody (bs-5615R)

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Anti-phospho-TRIM25 (Ser100) antibody

英文名称phospho-TRIM25 (Ser100)
中文名称磷酸化雌激素反应指状蛋白抗体
别    名TRIM25(phospho Ser100);TRIM25(phospho S100);EFP; Estrogen responsive finger protein; RNF 147; RNF147; TRI25; TRIM 25; Tripartite motif containing 25; Tripartite motif containing protein 25; Z147; Zinc finger protein 147; Zinc finger protein 147 (estrogen responsive finger protein); ZNF 147; ZNF147.   

DATASHEET

Host:Rabbit

Target Protein:phospho-TRIM25 (Ser100)

IR:Immunogen Range:AP(p-S)PN

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:7706

Swiss Prot:Q14258

Source:KLH conjugated Synthesised phosphopeptide derived from human TRIM25 around the phosphorylation site of Ser100:AP(p-S)PN 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc binding domains, a RING, a B box type 1 and a B box type 2, and a coiled coil region. The protein localizes to the cytoplasm. The presence of potential DNA binding and dimerization transactivation domains suggests that this protein may act as a transcription factor, similar to several other members of the TRIM family. Expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Pig
Cow
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Hela(Human) Cell Lysate at 30 ug
293T(Human) Cell Lysate at 30 ug
Primary: Anti- phospho-TRIM25 (Ser100) (bs-5615R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 71 kD
Observed band size: 71 kD

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