Anti-BNIP3 antibody
| 英文名称 | BNIP3 |
| 中文名称 | 促凋亡调节蛋白BNIP3抗体 |
| 别 名 | BCL2 Adenovirus E1B 19kDa Interacting Protein 3; BCL2/adenovirus E1B 19 kDa protein interacting protein 3; BCL2/adenovirus E1B 19 kDa protein-interacting protein 3; BNIP 3; BNIP3_HUMAN; NIP 3; NIP3. |
DATASHEET
Host:Rabbit
Target Protein:BNIP3
IR:Immunogen Range:101-194/194
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:664
Swiss Prot:Q12983
Source:KLH conjugated synthetic peptide derived from human BNIP3:101-194/194
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Apoptosis-inducing protein that, which can overcome BCL2 suppression. May play a role in repartitioning calcium between the two major intracellular calcium stores in association with BCL2.
Size:100ul
Concentration:1mg/ml
Applications:WB(1:500-2000)
ELISA(1:5000-10000)
Flow-Cyt(2ug/Test)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Blank control:A431.
Primary Antibody (green line): Rabbit Anti-BNIP3 antibody (bs-4239R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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