Anti-phospho-STAT5b (Ser731) antibody (bs-5703R)

Anti-phospho-STAT5b (Ser731) antibody

DATASHEET

Host:Rabbit

Target Protein:phospho-STAT5b (Ser731)

IR:Immunogen Range:AP(p-S)PA

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:6777

Swiss Prot:P51692

Source:KLH conjugated Synthesised phosphopeptide derived from human STAT5b around the phosphorylation site of Ser731:AP(p-S)PA 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:Carries out a dual function: signal transduction and activation of transcription. Binds to the GAS element and activates PRL-induced transcription.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
Flow-Cyt(1ug/Test)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Blank control (Black line):Molt4 (Black).
Primary Antibody (green line): Rabbit Anti-phospho-STAT5b (Ser731) antibody (bs-5703R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.