Anti-RAB21 antibody (bs-5830R)

Anti-RAB21 antibody 

DATASHEET

Host:Rabbit

Target Protein:RAB21

IR:Immunogen Range:51-150/225

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:23011

Swiss Prot:Q9UL25

Source:KLH conjugated synthetic peptide derived from human RAB21.:51-150/225 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:RAB21 belongs to the family of small GTP-binding proteins that regulate intracellular vesicles targeting. RAB21 regulates integrin internalization and recycling and it is essential for normal cell division and defects in this trafficking may contribute to multinucleation and genomic instability.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Cow
Horse
Rabbit
Sheep
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-RAB21 Polyclonal Antibody, Unconjugated(bs-5830R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining