Anti-MAS1 antibody (bs-5925R)

Anti-MAS1 antibody

DATASHEET

Host:Rabbit

Target Protein:MAS1

IR:Immunogen Range:51-150/325

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:4142

Swiss Prot:P04201

Source:KLH conjugated synthetic peptide derived from human MAS1:51-150/325 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The structure of the MAS1 product indicates that it belongs to the class of receptors that are coupled to GTP binding proteins and share a conserved structural motif, which is described as a '7 transmembrane segment' following the prediction that these hydrophobic segments form membrane spanning alpha-helices. The MAS1 protein may be a receptor that, when activated, modulates a critical component in a growth regulating pathway to bring about oncogenic effects.

Size:100ul

Concentration:1mg/ml

Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
Flow-Cyt(1ug/Test)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
Sheep
Guinea Pig
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Blank control: HepG2.
Primary Antibody (green line): Rabbit Anti-MAS1 antibody (bs- 5925R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.