Anti-phospho-LLGL1 + LLGL2 (Ser650+Ser654) antibody
| 英文名称 | phospho-LLGL1 + LLGL2 (Ser650+Ser654) |
| 中文名称 | 磷酸化相似肿瘤抑制基因HUGL抗体 |
| 别 名 | LLGL1 + LLGL2 (phospho S650 + S654); LLGL1 + LLGL2 (phospho Ser650 + Ser654); p-LLGL1 + p-LLGL2 (S650 + S654); p-LLGL1/LLGL2 (Ser650 + Ser654); p-LLGL1/LLGL2 (S650 + S654); p-LLGL1/LLGL2 (Ser650/654); DLG4; HGL; HUGL; HUGL1; lethal giant larvae homolog 1; lethal giant larvae homolog 2; Lethal(2) giant larvae protein homolog 1; Lethal(2) giant larvae protein homolog 2; LGL2; LLGL; L2GL1_HUMAN; L2GL2_HUMAN. |
DATASHEET
Host:Rabbit
Target Protein:phospho-LLGL1 + LLGL2 (Ser650+Ser654)
IR:Immunogen Range:PL(p-S)RVK(p-S)
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:3996
Swiss Prot:Q15334
Source:KLH conjugated synthesised phosphopeptide derived from mouse LLGL1 around the phosphorylation site of Ser650+Ser654:PL(p-S)RVK(p-S)
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:LLGL1 is a protein that is similar to a tumor suppressor in Drosophila. The protein is part of a cytoskeletal network and is associated with nonmuscle myosin II heavy chain and a kinase that specifically phosphorylates this protein at serine residues. The gene for LLGL1 is located within the Smith-Magenis syndrome region on chromosome 17. LLGL2 is a protein similar to lethal giant larvae of Drosophila. In fly, the protein's ability to localize cell fate determinants is regulated by the atypical protein kinase C (aPKC). In human, this protein interacts with aPKC-containing complexes and is cortically localized in mitotic cells.
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Guinea Pig
k.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-LLGL1+LLGL2(Ser650+Ser654) Polyclonal Antibody, Unconjugated (bs-6070R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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