Anti-RASGEF1A antibody
英文名称 | RASGEF1A |
中文名称 | 鸟嘌呤核苷酸交换因子rasgef1a抗体 |
别 名 | CG4853 gene product; Ras-GEF domain-containing family member 1A; RasGEF domain family, member 1A; rasgef1a; RGF1A_HUMAN; RASGEF 1A; RASGEF1A. |
DATASHEET
Host:Rabbit
Target Protein:RASGEF1A
IR:Immunogen Range:301-200/481
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:221002
Swiss Prot:Q8N9B8
Source:KLH conjugated synthetic peptide derived from human RASGEF1A:301-200/481
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:Guanine nucleotide exchange factor (GEF) with specificity for RAP2A, KRAS, HRAS, and NRAS (in vitro). Plays a role in cell migration.
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Guinea Pig
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-RASGEF1A Polyclonal Antibody, Unconjugated(bs-6156R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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