Anti-NCKAP1 antibody (bs-6204R)

Anti-NCKAP1 antibody

DATASHEET

Host:Rabbit

Target Protein:NCKAP1

IR:Immunogen Range:1001-1132/1132

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:3071

Swiss Prot:P55160

Source:KLH conjugated synthetic peptide derived from human 12 NCKAP1:1001-1132/1132 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:NAP125, also known as NCKAP1 (NCK-associated protein 1), p125Nap1 or membrane-associated protein HEM-2, is a 1,128 amino acid single pass membrane protein that exists as two alternatively spliced isoforms and belongs to the HEM-1/HEM-2 family. While widely expressed, NAP125 is found at highest levels in heart, brain and skeletal muscle where it regulates Rac-dependent actin remodeling as part of a lamellipodial complex with WAVE2, Abi-1 and CYFIP1. NAP125 localizes to the cytoplasmic side of lamellipodium membrane and is encoded by a gene that maps to human chromosome 2q32.1 and mouse chromosome 2 C3. NAP125 expression is markedly reduced in Alzheimer disease (AD)-affected brains, suggesting a possible role in the disease.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)

Cross Reactive Species:Human
Mouse
Rat
Chicken
Dog
Pig
Cow
Horse
Guinea Pig
G.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:
Skeletal muscle (Mouse) Lysate at 40 ug
Primary: Anti- NCKAP1 (bs-6204R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 129 kD
Observed band size: 129 kD

Blank control: HL60.
Primary Antibody (green line): Rabbit Anti-GPR65 antibody (bs-6204R-AF488)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20,000 events was performed.