Anti-phospho-BCAR1 (Tyr410) antibody
| 英文名称 | phospho-BCAR1 (Tyr410) |
| 中文名称 | 磷酸化乳腺癌抗雌激素耐药蛋白1抗体 |
| 别 名 | BCAR1 (phospho Y410); BCAR1(phospho Y410); p-BCAR1(p-Tyr410); Breast cancer anti estrogen resistance 1 protein; BCAR 1; Bcar1; BCAR1_HUMAN; Breast cancer anti estrogen resistance 1; Breast cancer anti-estrogen resistance protein 1; CAS; Cas scaffolding protein family member 1; Crk associated substrate; Crk associated substrate p130Cas; CRK-associated substrate; CRKAS; P130CAS. |
DATASHEET
Host:Rabbit
Target Protein:phospho-BCAR1 (Tyr410)
IR:Immunogen Range:GV(p-Y)AV
Clonality:Polyclonal
Isotype:IgG
Entrez Gene:9564
Swiss Prot:P56945
Source:KLH conjugated Synthesised phosphopeptide derived from human BCAR1 around the phosphorylation site of Tyr410:GV(p-Y)AV
Purification:affinity purified by Protein A
Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Background:p130 represents one of several known substrates for v-Crk encoded p47. p130 Cas (for Crk-associated substrate) exhibits a high level of tyrosine phosphorylation and is tightly associated with v-Crk, suggesting a role in v-Crk-mediated cell signaling. The molecular cloning of p130 Cas has shown it to represent a novel SH3 containing signaling molecule with a cluster of multiple putative SH2-binding motifs for v-Crk. By immunoprecipitation analysis, p130 Cas has been shown to be highly phosphorylated at tyrosine residues subsequent to either v-Src p60 or v-Crk-mediated transformation and to form stable complexes with both of these transforming proteins. p130 Cas behaves as an extremely potent substrate for protein tyrosine kinases and has been reported to relocate from the cytoplasm to cell membrane upon tyrosine phosphorylation. One proposed model is that the SH2 domain of v-Crk functions to activate c-Src kinase, which in turn phosphorylates p130 Cas.
Size:100ul
Concentration:1mg/ml
Applications:ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)
Cross Reactive Species:Human
Mouse
Rat
Horse
.
For research use only. Not intended for diagnostic or therapeutic use.
VALIDATION IMAGES
Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-BCAR1(Tyr410) Polyclonal Antibody, Unconjugated(bs-6224R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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