Anti-STK39 antibody (bs-2971R)

Anti-STK39 antibody

DATASHEET

Host:Rabbit

Target Protein:STK39

IR:Immunogen Range:201-330/545

Clonality:Polyclonal

Isotype:IgG

Entrez Gene:27347

Swiss Prot:Q9UEW8

Source:KLH conjugated synthetic peptide derived from human PASK/STK39:201-330/545 

Purification:affinity purified by Protein A

Storage:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Background:The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including cell division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/threonine (Ser/Thr) protein kinases. SPAK, also known as STK39 (serine threonine kinase 39), DCHT or PASK, is a 547 amino acid protein that localizes to both the cytoplasm and the nucleus and contains one protein kinase domain. Expressed predominately in pancreas, brain, heart, lung, liver and testis, SPAK functions as a Ser/Thr protein kinase that catalyzes the ATP-dependent phosphorylation of target proteins and is thought to be involved in mediating stress-activated signals. The gene encoding SPAK maps to human chromosome 2, which houses over 1,400 genes and comprises nearly 8% of the human genome.

Size:100ul

Concentration:1mg/ml

Applications:WB(1:500-2000)
ELISA(1:5000-10000)
IHC-P(1:100-500)
IHC-F(1:100-500)
IF(1:100-500)

Cross Reactive Species:Human
Mouse
Rat
Dog
Pig
Cow
Horse
Rabbit
.

For research use only. Not intended for diagnostic or therapeutic use.

VALIDATION IMAGES

Sample:293T cell (Human) Lysate at 40 ug
Primary: Anti-STK39(bs-2971R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 60kD
Observed band size: 60kD